Tissue Culture Room

TCR Bench

Equipment and Supplies

Core-Provided Equipment
  • Three Class II certified biosafety cabinets*
  • Four shared incubators with three shelves each, 5% CO2, and a 37°C humidity-controlled chamber with sterilized deionized water
  • Tabletop centrifuge with protective caps for infectious material**
  • Inverted compound microscopes
  • Automated cell counter***
  • Vacuum trap system for liquid waste
  • Water bath at 37°C
  • Upright standard fridge (4°C) to store cell media
  • Freezer (-20°C) to store assay reagents
  • Bins for biohazardous waste and sharps containers for any glass pipettes or vials
  • Small biohazard waste bags for pipette tips

*70% ethanol spray bottles, Kimwipes, electric pipettors, and micropipettes are available

**There is only one rotor available with swinging buckets and it is limited to spinning 15ml and 50ml centrifuge tubes

***Glass hemocytometer slides are also available

Core-Provided Materials
  • Micropipette tips (10, 200, and 1000 uL) 
  • Microcentrifuge tubes (0.5 and 1.5 mL)
  • Centrifuge tubes (15 and 50 mL)
  • Culture flasks (T75 and T175)
  • Filter bottles for media preparation (500 mL)
  • Gloves (S, M, L)
  • Bleach
  • Reagent Reservoirs
  • Cell counting slides / Trypan Blue for automated cell counter.
  • Liquid nitrogen storage

The user needs to provide any other material not mentioned in the lists above (this includes, but is not limited to: Cell lines, cell media, assay plates, assay reagents, PPE, etc.)